Success: Fin clipping!
Challenge: Creating master mixes
Question: What does the lysis buffer do ?
Zebrafish- Danio rerio
Lab update!
Lately, I have been working in the fish facility of the laboratory. I have been genotyping! I gather fish from a specific mutant tank named: 08bdth. I anesthesize the fish, clip their tails, and run the lysis buffer. Then I create master mixes for each stock solution: Afterwards, I add 1 mL of DNA and 19 mL of each master mix. PCR Is the final step then I begin to run the gel!
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