50 Questions pertaining my experiment:
- What mutant am I working with?
- How many phenotypes are visible?
- How many Wildtype Zebrafish are in each tank?
- Why do only the females matter in the beginning?
- Why am I studying this specific mutant?
- What purpose does Methanol serve in each well?
- How long can the Zebrafish survive in Tricaine?
- How long does it take for a fin to grow back after clipping?
- Does the fish die as soon as part of the tissue is accidentally clipped?
- How much DNA is used from the fin snip?
- What are the genes of each Zebrafish?
- What are the genes of each tank on an average?
- How many are homozygous?
- How many are heterozygous?
- How many are recombinant?
- How do we know when we are closer to the marker?
- How many genes per marker?
- How many genes per map?
- How many markers per map?
- Once one gene is found on a marker, am I closer to the next gene or marker?
- What is PCR?
- What is denaturation?
- What is annealing?
- What is extension?
- Why is the standard temperature 94 degrees Celsius?
- What does the lysis buffer do?
- How is lysis tested?
- How much of lysis is needed to run a test?
- What is Taq?
- What is the 10x PCR buffer's purpose?
- What does the dNTP solution do?
- Why is the gel in 3%?
- What is agarose?
- What is the purpose of using agarose for the gel?
- What is the purpose of Ethidium Bromide?
- Why is Ethidium Bromide highly toxic?
- What is the loading buffer?
- How do the molecules become negative?
- What is 100bp DNA ladder?
- How does the gel make the poles attract?
- What is each band on the DNA ladder?
- What is the measurement between each band?
- What does one band indicate?
- What do two bands indicate?
- How is a wildtype visible?
- How is a recombinant known?
- How long does the DNA sit for?
- What is the maximum time the DNA is "good" for?
- How do I know when I found the closest distance between each marker?
- How many genes per marker?
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