Fishing for the Gene- Narrowing the interval to identify the molecular nature of a new recessive maternal-effect gene
Maria Isaev
Central High School, Philadelphia, Pennsylvania
Ms. Galeet Cohen, teacher/ Ricardo Fuentes, mentor/ Dr. Mullins, sponsor
The 08bdth/spotty maternal-effect mutant was isolated and mapped after a four-generation crossing strategy of chemically-mutated zebrafish. By the use of Simple Sequence Length Polymorphic (SSLP) markers, the 08bdth/spotty gene was mapped on chromosome 2 between markers z24206 at 45.5 cM (centiMorgans) and z17291 at 52.4 cM. In this project, I focus on narrowing the genetic and physical interval containing the 08bdth/spotty gene by finding new genetic markers positioned closer to the mutation. The two questions I am asking are: What is the molecular identity of the 08bdth/spotty gene located on chromosome 2 and how could spotty regulate cytoplasmic segregation during Zebrafish early development. Narrowing the DNA interval will allow me to identify the molecular nature of a new recessive maternal-effect gene, the 08bdth/spotty gene, which regulates cytoplasmic segregation during zebrafish early development. After finding recombinants between markers z24206 and z17291, I have concluded that the gene is located closer to market z24206.
No comments:
Post a Comment