This week is a short week, therefore I will be focusing on organizing the laboratory freezing and creating my individual box of materials for my PCR reaction. This will make it easier and quicker to begin my PCR. The individual box will include a lysis buffer, stock solutions for each marker, F+R solutions for each marker, 10x PCR Buffer, DNTP solution, etc.
Success: A scientist must always stay prepared to ensure that they have no experimental flaws, which is why organization is key to an effective project.
Challenge: There are over 100 different solutions in the biology lab freezer! Finding every necessary sample size solution took focus.
Question: Why must the taq solution, necessary for PCR, be kept and lower temperatures than the rest?
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